All data in the plprot bell pepper chromoplast database are based on
shotgun protein identification by 1-D PAGE liquid chromatography
tandem mass spectrometry (LC-MS/MS). Raw data were interpreted by the
SEQUEST **software and searched against the NCBI non redundant protein
database (Viridiplantae subsection). SEQUEST assignments were manually
For details see Siddique et al. (Plant Cell Physiol., 2006 47: 1663-1673.).
For data search and output the relevant fields have to be checked.
Fields contain the following information:
- Organism: Since the Capsicum annuum genome is not completely sequenced, peptides must be identified on the basis of identical peptides form homologues proteins from different organisms in the database. Here, the organisms from which the identification originated is provided.
- Fraction: Serial extraction step from which the protein was identified. Abbreviations are: O- osmotic shock (soluble proteins), U- urea wash (peripheral membrane proteins) and S-SDS solubilization (integral membrane proteins)
- Localization: Prediction or reported function in plastids (Y- chloroplast transit peptide, reported pt- very close homologue has a reported function in plastids, co- putative contaminant)
- Protein: Protein annotation from the NCBI protein database
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